Method Development

The PulseNet network actively evaluates improvements in genotyping methodology and develops new methods. The network allows full validation of possible improvements to protocols to made using joint network resources.

PFGE Method Development

PFGE protocols are currently under development for

  • Vibrio vulnificus
  • Yersinia enterolitica
  • Campylobacter coli

PulseNet laboratories are also evaluating

  • Alternative agaroses for gel electrophoresis
  • Use of enzymes from different manufacturers
  • Alternative gel stains to use in place of ethidium bromide

For further information on PFGE protocol development please contact PulseNet USA.

MLVA method development

Protocols currently in development/validation include

  • Salmonella Enteriditis
  • Listeria monocytogenes
  • Shigells sonnei
  • Salmonella Newport
  • STEC non-O157

For further information on MLVA protocol development please contact Eija Trees.

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